Determination of Vanillylmandelic, 5-Hydroxyindoleacetic and Homovanillic Acid in Urine by Isocratic Liquid Chromatography

Direct measurement of homovanillic, vanillylmandelic and 5-hydroxyindoleacetic acids in urine by capillary electrophoresis.

Separation conditions in CE, with a neutral coated capillary and reversed polarity, have been optimised to make direct measurement of vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid possible in urine samples without pre-treatment. The method developed has been validated, presenting adequate parameters for linearity, accuracy and precision. Detection limits range from 0.0...

DEVELOPMENT OF A METHOD FOR THE DETERMINATION OF 4-HYDROXY-3-METHOXYMANDELIC ACID (VANILLYLMANDELIC ACID) IN URINE BY PAPER CHROMATOGRAPHY

We have developed a simple and precise paper chromatographic method for the determination of 4-hydroxy-3-methoxymandelic acid (VMA) in urine. Concentrations of VMA in patients with neuroblastoma were increased in comparison to controls. The linearity was excellent in the concentration range tested. The within-assay coefficient of variation for control and patient urine was less than 2.2%. ...

Uniform chromatographic conditions for quantifying urinary catecholamines, metanephrines, vanillylmandelic acid, 5-hydroxyindoleacetic acid, by liquid chromatography, with electrochemical detection.

Uniform liquid-chromatographic conditions were developed such that we could quantify norepinephrine, epinephrine, normetanephrine, metanephrine, vanillylmandelic acid, and 5-hydroxyindoleacetic acid in urine by using a single mobile phase of monochloroacetic acid and citric acid, 0.1 mol/L each. All compounds were separated on a C18 column and detected electrochemically at a potential of +0.800...

Cerebrospinal homovanillic acid and 5-hydroxyindoleacetic acid in huntington's chorea.

Specific photometric determination of 5-hydroxyindoleacetic acid in urine.

Udenfriend ‘s nitrosonaphthol test for 5-hydroxyindoleacetic acid (5-HIAA) is subject to interference by urinary phenolic acids and drug metabolites. Studies in our laboratory have shown that the specificity and sensitivity of the nitrosonaphthol reaction with 5-hydroxyindoles can be greatly enhanced by the addition of 2-mercaptoethanol. This observation serves as the basis of a simple, improve...